Monkey Primary Aortic Fibroblasts
Model: MK-6075
Product Description
Cynomolgus Monkey Primary Aortic Fibroblasts from Cell Biologics are isolated from Cynomolgus Monkey Aortic tissue. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.
Product Testing
Cynomolgus Monkey Primary Aortic Fibroblasts from Cell Biologics are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Cynomolgus Monkey Primary Aortic Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
Cynomolgus Monkey Primary Aortic Fibroblasts from Cell Biologics are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Suggested Medium: Fibroblast Medium /w Kit –500 ml
Catalog No. M2267
C57BL/6 Mouse Primary Aortic Endothelial Cells
Model: C57-6052
Product Description
C57BL/6 Mouse Primary Aortic Endothelial Cells from Cell Biologics are isolated from the aortic tissues of pathogen-free laboratory mice. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains 1×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Product Testing
C57BL/6 Mouse Primary Aortic Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, from Santa Cruz); AF1002 from R&D System or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, from BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
C57BL/6 Mouse Primary Aortic Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
C57BL/6 Mouse Primary Aortic Endothelial Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution, for appropriate endothelial cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Suggested Medium: Endothelial Cell Medium /w Kit –500 ml
Catalog No. M1168
C57BL/6 Mouse Bone Marrow Macrophages (Frozen Cells)
Model: C57-6030F
C57BL/6 Mouse Bone Marrow Macrophages- Frozen Cells
Catalog No. C57-6030F
Suggested Medium: Macrophage Medium /w Kit (500 ml)
Catalog No. M3368
Product Description
C57BL/6 Mouse Bone Marrow Macrophages are derived from the tibias and femurs of pathogen-free laboratory adult mice. C57BL/6 Mouse Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in Cell Biologics’ Culture Complete Growth Medium for 5-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 6 x106 cells and is delivered frozen. C57BL/6 Mouse Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. C57BL/6 Mouse Bone Marrow Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. They can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics.
Laboratory Applications
C57BL/6 Mouse Bone Marrow Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics Products
C57BL/6 Mouse Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics' Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Although C57BL/6 Mouse Bone Marrow Macrophages are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Antibiotic/Antimycotic Solution (1:100)
Model: 6920
Antibiotic-Antimycotic Solution – 100 ML (100X)
Catalog Number: 6920
Description
Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Amphotericin B is used to prevent fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeas.
Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.
Formulation
Contains 10,000 units of penicillin (base), 10,000 ug of streptomycin (base), and 25 ug of amphotericin B/ml utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Antimycotic in 0.85% saline
Storage Condition
Store the solution at -20°C.
Shipping
Ice Pack.
Authorized Uses of Cell Biologics Products
Antibiotic-Antimycotic Solution from Cell Biologics is distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
