Complete Mouse Embryonic Stem Cell Medium

Complete Mouse Embryonic Stem Cell Medium

Catalog Number: M6822

Description

Mouse Embryonic Stem Cell Medium is a complete medium designed for the culture of mouse embryonic stem cells and mouse induced pluripotent stem cells. It was tested and optimized with mouse embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) growth and proliferation in vitro. It is formulated for use with 5% CO₂ and 95% air in a humidified incubator. The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with fibroblast cell growth supplement, antibiotics, and fetal bovine serum. 15% Fetal Bovine Serum (germline transmission-tested) contains in the medium.

Cell Culture Medium Supplements include:

- 75.0 ML FBS

- 5.0 ML GlutaMAX™ Supplement

- 5.0 ML Penicillin-Streptomycin

- 1.0 ML 2-Mercaptoethanol

- 0.1 ML LIF

*Before use, add Supplements into 410 DMEM basal medium.

 Storage Condition

 Store 500 ml basal medium at 2-6°C. Store cell growth factors, supplements, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplements can be kept in 4°C for two months. Protect from light.

To assure sterility, the prepared medium should be sterilized by filtration through a 0.2 µm filter.

Shipping

Ice pack and dry ice.

Authorized Uses of Cell Biologics’ Products

Complete Cell Culture Media from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.