Canine Liver Kuffer Cells

Canine Liver Kuffer Cells

Catalog No. D-6226

Suggested Medium:  Macrophage Medium /w Kit (500 ml)

Catalog No. M3368

Product Description

Canine Liver Kuffer Cells are derived from the liver tissue of beagle dog. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Each vial contains 3 x10⁶ cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Canine Liver Kuffer Cells can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics Products

Canine Liver Kuffer Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics' Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the

Cells that they receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at

http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.