iNOS Knockout Mouse Primary Embryonic Fibroblasts

iNOS Knockout Mouse Primary Embryonic Fibroblasts

Catalog No.  iNOS-3028

Suggested Medium: M2267 Mouse Fibroblast Medium /w Kit – 500 ML

Product Description

Mouse Primary Embryonic Fibroblasts from Cell Biologics are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Embryonic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.  Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen. Mouse Primary Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or flow cytometry. 

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice.  On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.  Live cell shipment is also available on request.

Authorized Uses of Cell Biologics’ Products

Mouse Primary Embryonic Fibroblasts from Cell Biologics are distributed for research purposes only.  Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures.  Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent.  Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaim

Although Mouse Primary Embryonic Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

CELL BIOLOGICS’ guarantee applies only to your purchase of CELL BIOLOGICS' cells with CELL BIOLOGICS’ Media and Coating Solution for appropriate cell culture and cell testing following CELL BIOLOGICS’ online protocols within 35 days from the date of product delivery.