iPS-Derived Endothelial Cell Differentiated Medium

iPS-Derived Endothelial Cell Differentiated Medium

Catalog Number: M6861

Description

iPS-Derived Endothelial Cells Differentiated Media designed for the differentiation of iPS into endothelial cells. Medium contains growth factors and supplements. It is formulated for use with 5% CO₂ and 95% air in a humidified incubator. The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with cell growth supplement, antibiotics, and fetal bovine serum.

 Cell Culture Medium and Supplements include:

-       125 ML Ham's F-12 Nutrient Mix

-       375 ML  IMDM

-      2.5 ML N2-SUPPLEMENT

-       5.0 ML B-27 Supplement Minus

-       2.5 ML 10%BSA

-       0.5 ML MTG (1-Thioglycerol,0.45M,1000x)

-       0.5 ML Ascorbic acid (50mg/ml, 1000x)

*Before use, add growth factors into 100ml basal medium with supplements as following:

 -       0.1 ML VEGF

-       20 ul  FGF-2 

-       4 ul BMP-4  

Storage Condition

Store 500 ml basal medium at 2-6°C. Store cell growth factors, supplements, fetal bovine serum (FBS) at -20°C. The complete cell culture medium with Supplements can be kept in 4°C for 2-4weeks. Protect from light.

To assure sterility, the prepared medium should be sterilized by filtration through a 0.2 µm filter.

Shipping

Ice pack and dry ice.

Authorized Uses of Cell Biologics’ Products

Complete Cell Culture Media from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.