DMEM/Ham's F-12 (1:1 Mixture), With L-glutamine and HEPES

Description

Since Harry Eagle's first reports, studies have been underway to determine the media requirements for individual cell types. The medium found to be most satisfactory for these studies as a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. To help compensate for the buffering capacity lost with the removal of serum, HEPES has been added in some modifications at a final concentration of 15 mM.

D-Glucose 3151mg/L
L-Glutamine 365mg/L
HEPES 3574.5mg/L
Sodium Pyruvate 55mg/L
Phenol Red 8.1mg/L

Storage Condition

Store the basal medium at 2-8°C for 8 months. Protect from light.

Note: To assure sterility after 2 weeks or if there is concern that sterility was compromised during the supplementation process, the prepared medium may be refiltered with a 0.2 um filter.

Shipping

Ice pack.

Authorized Uses of Cell Biologics Products

Cell Media from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.