Human Pancreatic Islets

Human Pancreatic Islets

Catalog No.  H-6232L (Live Cells); H-6232F (Frozen Vial)

Suggested Medium: Pancreatic islet culture medium /w Kit – 500 ML

Catalog No. M2269

Product Description

Human Pancreatic Islets from Cell Biologics are isolated from the pancreas of human using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in Cell Biologics’ Pancreatic Islet Culture Medium containing 5% FBS.

Product Testing

Human Pancreatic Islets are tested for their viability using FDA/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.

Laboratory Applications

Human Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO₂ incubator.

Authorized Uses of Cell Biologics’ Products

Human Pancreatic Islets from Cell Biologics are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Primary Cell Culture Protocol

• All cell culture procedures must be conducted in a bio-safety cabinet.
• Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.
• Use aseptic technique to prevent microbial contamination.
• Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.

Medium: 

• Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.
• Handling of Arriving Live Cells
• When you receive the live cells in a tube, keep cells in 37°C CO₂ incubator for 15 min.
• Centrifuge cells at 500 rpm for 1 minute.
• Aspirate the supernatant and resuspend the cell pellet in 6 ml of Cell Biologics’ Cell Culture Growth medium (Catalog No. M2269).
• Transfer cells from a vial to the cell culture plate.
• Place a plate in a humidified, 5%-CO₂ incubator at 37°C until experiments.
• Cells can be cultured for 3-7 days.
• Change fresh cell culture medium every 24-48 hours.
• Cells should be checked daily under a microscopy to verify appropriate cell morphology.