Mouse Tumor-Associated Endothelial (Human Prostate Cancer Origin, MTPSECH)

Product Description

Mouse Tumor-Associated Endothelial Cells (Human Prostate Cancer Origin, MTPSECH) from Cell Biologics were isolated from pathogen-free laboratory nude mice. Cells were isolated from human xenograft of pathogen-free laboratory nude mice. Human prostate adenocarcinoma epithelial cells (PC-3) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTPSECH were cultured in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. Cells will be delivered in a frozen vial and each vial contains 1x10⁶ cells/ml. The method we use to isolate primary tumor-associated endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

MTPSECH are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, from Santa Cruz); AF1002 from R&D System or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, from BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

MTPSECH can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution, for appropriate endothelial cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Suggested Medium: Endothelial Cell Medium /w Kit – 500 ml

Catalog No. M1168