Rat Primary Alveolar Macrophages

Rat Primary Alveolar Macrophages

Catalog No.  RA-2313

Suggested Medium:  M3368 Complete Macrophages Medium /w Kit (Animal) – 500 ml

Product Description

Rat Priamry Alveolar Macrophages are derived from the lung of 6-8 weeks old Sprague–Dawley Rats. Each vial contains at least 1 x10⁶ cells. Rat Alveolar Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Rat Alveolar Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. 

Laboratory Applications

Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products 

Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180°C) until ready for experimental use. Live-cell shipment is also available on request.

Authorized Uses of Cell Biologics’ Products

Rat Alveolar Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

CELL BIOLOGICS’ guarantee applies only to your purchase of CELL BIOLOGICS' cells with CELL BIOLOGICS’ Media and Coating Solution for appropriate cell culture within 35 days from the date of product delivery.

General protocol for the culture of primary cells

All cell culture procedures must be conducted in a bio-safety cabinet. Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter. Use aseptic technique to prevent microbial contamination.

Recommended Cell Seeding:

 0.7-0.8 million cells are seeded per well of a 12-wll plate or 1-1.5 million macrophages are seeded per well of a 6-well plate.

• Place a plate in a humidified, 5%-CO₂ incubator at 37°C until experiments.
• Change fresh cell culture medium every 24-48 hours.
• Cells should be checked daily under a microscopy to verify appropriate cell morphology.