Sprague-Dawley Rat Primary Spleen Endothelial Cells
- Product Code: RA-6057
- Availability: In Stock
Product Description
Sprague-Dawley Rat Primary Spleen Endothelial Cells from Cell Biologics are isolated from spleen tissue of 6-8 week old laboratory Sprague-Dawley rat. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Product Testing
Sprague-Dawley Rat Primary Spleen Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, from Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, from Life Technologies) by immunofluorescence staining. Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, from Invitrogen), a functional marker for endothelial cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Sprague-Dawley Rat Primary Spleen Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
Sprague-Dawley Rat Primary Spleen Endothelial Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution, for appropriate endothelial cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Suggested Medium: Endothelial Cell Medium /w Kit – 500 ml
Catalog No. M1266