Dog Liver Endothelial Cells
- Product Code: D-6017
- Availability: In Stock
Dog Liver Sinusoidal Endothelial Cells
Catalog No. D-6017
Suggested Medium: M1266 Endothelial Cell Medium /w Kit – 500 ML
Product Description
Dog Liver Endothelial Cells from Cell Biologics are isolated from tissue of dog liver. Dog Liver Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Product Testing
Dog Liver Endothelial Cells are tested for expression of markers using antibody, Ve-cadherin (CD144, VE-cadherin Anitbody, C-19, sc6458 Santa Cruz) by immunofluorescence staining. Dog Liver Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics.Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Dog Liver Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry.
Storage of Cell Biologics Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics Products
Dog Liver Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biolgics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
CELL BIOLOGICS’ guarantee applies only to your purchase of CELL BIOLOGICS' cells with CELL BIOLOGICS’ Media and Coating Solution for appropriate cell culture and cell testing following CELL BIOLOGICS’ online protocol within 35 days from the date of product delivery.