MEM With Earle's Salts and L-glutamine

MEM With Earle's Salts and L-glutamine 

Catalog No. M6211

A 6 bottles minimum is requied per order.

Description

Minimum Essential Medium (MEM) was developed by Harry Eagle and has become one of the most widely used of all synthetic cell culture media. The original formulation, containing Earle's Balanced Salts, was designed for use in propagating mouse L cells and HeLa cells. Eagle found that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM with Earle's Balanced Salts is suitable for cell culture in CO₂ charged atmospheres, while MEM with Hank's Salts is suitable for cell culture in normal atmospheric conditions.

D-glucose--------- 1000mg/L
L-Glutamine------  292mg/L

Storage Condition

Store the basal medium at 2-8°C. Store endothelial cell growth supplement, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplement Kit can be kept in 4°C for two months.  Protect from light.

Note: To assure sterility after 2 weeks or if there is concern that sterility was compromised during the supplementation process, the prepared medium may be refiltered with a 0.2 um filter.

Shipping

Ice pack and dry ice.

Authorized Uses of Cell Biologics Products

Cell Media from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures.  Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.